Chemical structural features and anti-complementary activity of polysaccharide HPS1-D from Hedysarum polybotrys / 中国中药杂志

HPS1-D, an active polysaccharide,was isolated and purified from Hedysarum polybotrys. HPS1-D was obtained after treated with Savage method and H2O2, and purified with DEAE-cellulose 52 and Sephadex G-100 gel filtration chromatography. Then physicochemical property analysis, GC, methylation, partial acid hydrolysis, and NMR method were used to study chemical structural of HPS1-D. The conformation was primarily analyzed with GPC-MALLS method and Congo red reaction. The anti-complementary activity of HPS1-D was evaluated with the hemolysis assay. HPS1-D was a heteropolysaccharide and consisted of D-glucose, L-arabinose, (7.2:1.3). HPS1-D proved to be a neutral sugar, with 1, 4-and 1, 4, 6-alpha-D-glucopyranosyl residues in backbone ,and 1, 5-and 1, 3, 5-alpha-L-arabinofuranosyl residues in branches. HPS1-D has a random coil state conformation with monodisperse mass distribution in 0.9% NaCl solution. And HPS1-D had triple-helix conformation in concentrate of NaOH solution. Anti-complementary activity of HPS1-D was closed to its positive control heparin.

Spectrum-effect relationship of active fraction from Angelicae Sinensis Radix with effect of reinforcing Qi / 中草药

Objective: To analyze the correlation between HPLC chromatogram and the effect of different extracted fractions from Angelicae Sinensis Radix (ASR) on reinforcing Qi. Methods: Qi deficiency model of mice was set up by controlling diet and fatigue. The influences of the different extracted fractions from ASR on phagocytotic ability of mononuclear macrophage and immune organs of mice with the symptoms of Qi deficiency were compared, and the active fraction of ASR was screened. The part which was extracted with 70% ethanol (ethanol extract) from ASR has the certain improvement on the Qi deficiency symptoms. The peak areas of each common peak from HPLC fingerprint were correlated with the pharmacodynamic efficacy, and the grey relation statistics was used to study the spectrum-effect relationship. Results: The contribution of various components in ethanol extract with the effect of reinforcing Qi was determined by the spectrum-effect relationship, the order was as follows (characteristic peak number): 18 > 5 > 7 > 12 > 17 > 4 > 19 > 2 > 20 > 6 > 8 > 3 > 14 > 1 > 16 > 11 > 9 > 13 > 15 > 10, peak 7, 10, 11, 13, 17, 18, and 20 are ferulic acid, senkyunolide I, senkyunolide H, senkyunolide A, ligustilide, butylidenephthalide, and levistilide A, respectively. Conclusion: The ethanol extract of ASR has the strong activity of reinforcing Qi, and there is a certain correlation between HPLC specific chromatogram and the effect.

Investigation on chromatogram-pharmacodynamics relationship of Angelica sinensis on effect of replenishing blood / 中国中药杂志

Blood deficiency model of mice was copied by subcutaneous injection with 200, 100 and 100 mg x kg(-1) (0.01 mL x g(-1)) acetyl phenylhydrazine (APH) at the frist, fourth, and seventh days. Mice in each group were perfused with different extracted parts of Angelica sinensis (drug dosage was 2.4 g x kg(-1)) at the tenth day, once a day for 10 days. Then compare the influence of different extracted parts of Angelica sinensis to RBC, Hb, PLT and thymus, spleen and weight changes of blood deficiency mice. The peak areas of each common peak from HPLC fingerprint were associated with the date of replenishing blood pharmacodynamics efficacy by using gray relation statistic, which was used to research the chromatogram-pharmacodynamics relationship. The results showed that the part of DSC has the better effect in replenishing blood. The contribution degree of the DSC to replenishing blood of each component were determined by correlation size, and ferulic acid made the largest contribution, but contribution of other components should not be ignored. In this paper, we research the relationship of the HPLC fingerprint and spectrum activity relationship, determine the material basis of the DSC for replenishing blood, and provide effective way to represent the spectral correlation effect.

Sulfated modification and anticoagulant activity in vitro of sulfated glucan isolated from the aqueous extract of Hedysarum polybotrys / 药学学报

SHG was sulfated by chlorosulfonic acid-pyridine method, and six samples which we got were prepared in different reaction conditions. There is a characteristic absorption peak near 260 nm in UV spectra and there are two characteristic absorption peaks near 1240 cm(-1) and 810 cm(-1) in the FT-IR. Degree of sulfation (DS) was calculated by elemental analysis and turbidimetry. Under the same conditions the absorption peaks become strong with the DS increase. The anticoagulant activity of SHG and sulfated modification samples was evaluated by the classic coagulant assays of prothrombin time (PT), activated partial thrombin time (APTT) live enzymes, and plasma thrombin time (TT). Results show that sulfated SHG has a good anticoagulant activity in vitro, and DS increased activity within a certain range.